Journal: Journal of Neurophysiology

Article Title: Mesoscale-duration activated states gate spiking in response to fast rises in membrane voltage in the awake brain

doi: 10.1152/jn.00116.2017

Figure Lengend Snippet: Anatomical and electrophysiological validation of recordings. A: fluorescence micrograph of CA1 showing the location of the tip of the LFP electrode (yellow; residual DiI left by a glass electrode, indicated with red arrow), the path and final location of the patch electrode (green; whole cell recording location indicated with green arrow), and DAPI staining (blue). B: representative LFP recordings showing theta oscillations, with gamma oscillations nested in them [left; raw trace in black, bandpass-filtered (6–12 Hz) trace in green] and sharp-wave ripples [SWRs, right; raw trace in black, bandpass-filtered (150–250 Hz) trace in red]. C: simultaneous LFP recordings at the LFP electrode location and at the target location for whole cell recordings (left) and the cross-correlation between the 2 traces (right; >0.9 with zero lag). D: biocytin-filled CA1 pyramidal neuron (left) and biocytin-filled pyramidal neuron in barrel cortex (right). Scale bars, 50 μm.

Article Snippet: We characterized these intermediate duration (or mesoscale) rises in the mouse barrel cortex using simultaneous dual whole cell patch clamp (which can be performed in surface structures by our multineuron autopatching system) and found that, for any pair of neurons, they occurred in a coordinated fashion at least part of the time, suggesting that they are shared by time-varying subsets of neurons within the network.

Techniques: Biomarker Discovery, Fluorescence, Staining